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Cardiac Gene Expression: Methods and Protocols (Methods in Molecular Biology)

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This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. The concluding section of the book highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

The past decade has ushered in enormous changes in how we perceive and study changes in gene expression in the heart. Early in the 1990s, the human genome project was just getting underway and establishing methods with the sensitivity to measure changes in the expression of genes with low copy number was an accomplishment. We all experienced some trepidation when the first news of microarrays arrived espousing the ability to measure changes in expression of hundreds to tens of thousands of genes (the whole genome) at once. This high throughput method was an astonishing jump in our approach to biological science. At the same time Steve Fodor and Pat Brown published papers describing two completely different approaches to measuring the expression changes of large numbers of genes at the same time. Thus began the microarray era and as a consequence the beginning of an era with a host of new approaches in pursuit of understanding the role and regulation of gene expression in cell biology and pathology including driving forward the field of bioinformatics.

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Beyond Buds: Marijuana Extracts—Hash, Vaping, Dabbing, Edibles and Medicines
Beyond Buds: Marijuana Extracts—Hash, Vaping, Dabbing, Edibles and Medicines
Beyond Buds is a handbook to the future of marijuana. Prohibition’s end has led to a technological revolution that’s generated powerful medicines and products containing almost zero carcinogens and little smoke. Marijuana icon Ed Rosenthal and leading cannabis reporter David Downs guide readers through the best new...
Computers & Typesetting, Volume A: The TeXbook
Computers & Typesetting, Volume A: The TeXbook
This guide to computer typesetting using TeX is written by Donald Knuth, the system's creator. TeX offers both writers and publishers the ability to produce plain or technical text, especially text containing a great deal of mathematics, comparable to the work of the finest printers. Novice and expert alike will find The TeXbook useful; it is...
Microsoft(r) Visual C#(R) 2005 Express Edition: Build a Program Now!
Microsoft(r) Visual C#(R) 2005 Express Edition: Build a Program Now!
Visual C# 2005 Express and the other Visual Studio 2005
Express Edition products are, in my opinion, one of the best
and most intelligent ideas to come out from Developer
Division here at Microsoft. I’m applauding and cheering
for the people who had this brilliant idea because I believe
there is a real need and demand
...

Using FileMaker Bento
Using FileMaker Bento

Bento is a product from FileMaker, which is owned by Apple. Designed to bring FileMaker’s database expertise to users of Mac OS X Leopard and later, it integrates data from iPhoto, iCal, Mail, and Address Book with databases that you can create from your own data as well as data imported from other sources.

Bento is...

Object-Oriented Design with ABAP: A Practical Approach
Object-Oriented Design with ABAP: A Practical Approach

Conquer your fear and anxiety learning how the concepts behind object-oriented design apply to the ABAP programming environment.  Through simple examples and metaphors this book demystifies the object-oriented programming model.

Object-Oriented Design with ABAP presents a bridge from the familiar procedural style...
MPLS Configuration on Cisco IOS  Software
MPLS Configuration on Cisco IOS Software

MPLS Configuration on Cisco IOS Software is a complete and detailed resource to the configuration of Multiprotocol Label Switching (MPLS) networks and associated features. Through its practical, hands-on approach, you'll become familiar with MPLS technologies and their configurations using...

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