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CRISPR Guide RNA Design: Methods and Protocols (Methods in Molecular Biology, 2162)

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This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.
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Quantum Monte Carlo: Origins, Development, Applications
Quantum Monte Carlo: Origins, Development, Applications
Monte Carlo methods are a class of computational algorithms for simulating the behavior of a wide range of various physical and mathematical systems (with many variables). Their utility has increased with general availability of fast computers, and new applications are continually forthcoming. The basic concepts of Monte Carlo are both simple and...
The Encyclopedia of Addictive Drugs
The Encyclopedia of Addictive Drugs
The Encyclopedia of Addictive Drugs will save readers many hours of time that would otherwise be spent tracking down basic facts in science journals and libraries. This book is useful to a wide variety of persons—from a student doing a term paper to reporters preparing a story, from parents reading that story to a narcotics law enforcement...
How to Lie with Maps
How to Lie with Maps
Originally published to wide acclaim, this lively, cleverly illustrated essay on the use and abuse of maps teaches us how to evaluate maps critically and promotes a healthy skepticism about these easy-to-manipulate models of reality. Monmonier shows that, despite their immense value, maps lie. In fact, they must.

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Network Query Language with CDROM
Network Query Language with CDROM
Learn about an exciting new technology that is revolutionizing network and Internet content delivery

As the first pure language of the content engineering era, Network Query Language (NQL) is the ultimate tool for rapid and simple development of intelligent agents, bots, spiders, middleware, and scalable business-to-business content aggregation...

Liposuction: Principles and Practice
Liposuction: Principles and Practice

The contributors to this book have spent time and effort presenting the cosmetic and plastic surgeon with information on the techniques and uses of liposuction for cosmetic and non-cosmetic surgery purposes. This constitutes the first book on cosmetic and non-cosmetic liposuction. It provides a how-to-do manual for all procedures of cosmetic...

The Complete Idiot's Guide to the Internet
The Complete Idiot's Guide to the Internet
Easily accessible and fun overview of everything the Internet has to offerThe new edition weeds out some coverage of lesser used Internet features (such as Archie and Gopher) replacing them with hot up-to-date coverage of live audio, conferencing, and push mediaThe Complete Idiot's Guide to the Internet, 4th Edition is an updated version of...
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