A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.
This book is written for those who are about to use their fi rst home computer or
those who have yet to explore the wider opportunities afforded by a computer
beyond searching the Internet and sending the occasional email message. You
have my word that there will be very few acronyms and those that do appear will
be fully explained in...
"Rex Black has done it again!...Critical Testing Processes is a book that all software testers will want to read from cover to cover and then retain for frequent reference."
—From the Foreword by Rick Craig, software test manager, author, lecturer, and consultant
The latest edition of Java in a Nutshell is designed to help experienced Java programmers get the most out of Java 7 and 8, but it’s also a learning path for new developers. Chock full of examples that demonstrate how to take complete advantage of modern Java APIs and development best practices, the first section of this...
This book introduces methods of re-processing images to extract numerical information that can be used to quantify the observables in environmental modelling. Experiments or procedures that yield large images can be statistically or parametrically examined. Through the use of open source libraries, the book shows how ‘big data’...
