This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and...
Hacking is the art of creative problem solving, whether that means finding an unconventional solution to a difficult problem or exploiting holes in sloppy programming. Many people call themselves hackers, but few have the strong technical foundation needed to really push the envelope.
Rather than merely showing how to run existing exploits,...
Extending jQuery teaches you to build custom extensions to the jQuery library. It covers all the extension points within jQuery and provides guidelines and techniques on how to make use of them. You'll not only discover how to write plugins, but how to design them for maximum reuse. You'll...
Get the brutal truth about coding, testing, and project management—from a Microsoft insider who tells it like it is. I. M. Wright's deliberately provocative column "Hard Code" has been sparking debate amongst thousands of engineers at Microsoft for years. And now (despite our better instincts), we're making...
Anyone who interacts with today's modern databases needs to know SQL (Structured Query Language), the standard language for generating, manipulating, and retrieving database information. In recent years, the dramatic rise in the popularity of relational databases and multi-user databases has fueled a healthy demand for...
This book was born out of a desire to help teachers teach their students math by being engaged in its study, and by showing teachers how they can custombuild visual examples of some of the concepts they are trying to get across to their students. Microsoft Excel is an extremely powerful spreadsheet with literally hundreds of built-in mathematical,...
The modern world offers lots of readily available online resources for learning. Wikipedia,
Google, news sources, millions of Web sites and blogs, even YouTube, offer access to
information in nearly any subject that triggers your curiosity and interest. Nonetheless, I
continue to believe that for deep understanding of something, nothing...
Absolutely, says Stephen Young-especially when it comes to those critical behaviors that can make or break performance. The reason is simple: no matter what you think you're saying, your words, gestures, and tone of voice can actually communicate something entirely different.
Harness the power of Adobe InDesign's data merge and style panel. Whether you're creating custom mail-outs or other mail-merge needs, familiarize yourself with this powerful InDesign panel in this in-depth, step-by-step guide. This book shows you how to easily create, edit, and print data merged documents that match...
Ostensibly, you buy a computer or gadget to get something done. The something might be as
simple as listening to MP3s you’ve ripped from your CD collection or as challenging as creating
a full-length feature film. Apple is happy to sell you products to meet your needs. The trouble is
your needs aren’t...
Yes, it is possible to be all things to all people, if you're talking about the Emacs editor. As a user, you can make any kind of customization you want, from choosing the keystrokes that invoke your favorite commands to creating a whole new work environment that looks like nothing ever developed before. It's all in Emacs Lisp -- and...
With clear, concise, step-by-step instructions on how to create presentations, users can create multiple-choice tests that not only give feedback, but also keep score....This is a powerful tool for educators to have in their repertoire....This book will encourage PowerPoint users to push their skills beyond the status quo.
